This sop is written for instruments that measure temperature, ph, dissolved oxygen, specific conductance, turbidity, andor oxidationreduction potential orp and the probe readings for ph, dissolved oxygen, and specific conductance are automatically corrected for temperature. Operation of akta pure chromatography system buffer a. To change the custom buffer value in accordance with current temperature. This procedure describes how to accurately measure the ph of a solution. Standard operating procedures for measuring ph, conductivity, and. Standard operating procedure turbidity, turbidimeter. P01001a the calibration procedure for the jenway 3505 ph meter introduction ph is the scale of measurement of acidity or alkalinity in aqueous water based solutions. The ph meter objective to describe the procedure for operation of ph meter scope this sop is applicable for the operation of ph meter responsibility officer executive quality control accountability manager quality control procedure operation of ph meter 1 ensure that the instrument is on clean and dry platform. A good slope should be between 55 and 60 mvs while the ideal is 59 mv. Thank you for purchasing this high quality mettler toledo laboratory meter. The first source is caused by change in electrode output at various. It is applicable to ph meter installed in the quality control department.
Record the date the buffers are changed under notes on the form. Temperature effects on the electrometric measurement of ph arise from two sources. Maintenance and calibration of ph meter document no. Standard operating procedure for ozarks environmental and. Glp sops for equipment calibration and maintenance. Speci c attention is given to address the di erences in measuring solid and liquid samples. Standard operating procedures analysis of ph this guidance document was prepared to assist research assistants, postdocs and lab technicians in conducting analysis of ph in the umass environmental engineering research laboratories. Sop on issuance, preparation, storage, usage and disposal of disinfectant solution.
The samples are placed in a refrigerator in the laboratory. Document templates are valuable tools and standard operating procedure sop templates are no exception. Author name title signaturedate approver name title signaturedate. Natural waters generally have a ph value between 4 and 9 standard methods, 2005.
Sop for monitoring of temperature, relative humidity and differential pressure. The location where samples are collected, identified by a gps location. Omcl network of the council of europe quality management document pa ph omcl 86 3r. Urs user requirement specification vmp validation master plan sop standard operating procedure usp u. Missouri state university and ozarks environmental and. Standard operating procedure for missouri state university.
Record the ph value and temperature on the ph meter calibration check record form. Multiple ph slopes and offset display enables quick, easy electrode diagnosis. Sep 30, 2015 ph is the measure of a solutions hydrogen ion activity. With the new fiveeasy and fiveeasyplus bench line instruments for ph and conductivity measurement, we wish to simplify your. Perform at least a 2point calibration at room temperature using standard buffers that bracket one above and one below the expected sample range. Record the expiration dates of the buffers on the ph meter calibration record form see section 14. At level i of the qualification of a ph meter selection of instruments and suppliers it is. Msds material safety data sheets located in the guidance manual binder. The ph meter measures the voltage and uses a temperaturedependent factor to convert the voltage to ph. Use the procedures recommended by manufacturer of equipment this.
Users must ensure document is current prior to each use. Fiveeasy and fiveeasy plus are much more than just a series of bench meters with an excellent price. Snap the neck off an alkalinity ampule standard, 0. The purpose of this standard operating procedure sop is to establish all the necessary steps to use, store, and care for a ph electrode. Confirm the calibration by reading the ph of the ph 7 buffer a second time see 12.
Rinse the ph electrode with milliq water and gently blot dry with a laboratory tissue. Gently remove the protective capsleeve from the bottom of the ph electrode. This page contains the procedures and forms used by technical services in the monitoring and measuring devices process. Standard operating procedure measurement of ph chemsop 1. Standard operating procedure for electrometric ph cromlab.
Sujana adepartment of pharmaceutical analysis, andhra university. Take 20 ml hamilton buffer solution from buffer bottle in its measuring cup by pressing the bottle. Scope this standard operating procedure sop applies to the staff and students using the hanna benchtop ph meter hi 8521 in the laboratories of the pharmacy department, university of malta. In this method, a calibrated ph meter is used to measure the ph value for water samples in the laboratory. Copy the inf file from the software upgrade package to a convenient location on the pc. This document is the standard operating procedure sop for conducting field. Once you have a stable reading, set the ph meter to the buffers ph value by pressing measure a second time. The buffers selected should bracket the ph of the samples. Scope applicable to digital ph meters located in traces centre and ug laboratory. Anion exchange chromatography of green fluorescent protein gfp using the akta pure system 9.
You will be prompted to enter the distance from the adcp to the bank enter this value into the end edge dialog box. A neutral solution such as pure water has a ph of 7. Pharmacopoeia 4 process and equipment description the degree of acidity or. Standard operating procedure of calibration of ph meter using buffer solutions of 7. All the lcd segments display for a few seconds as the meter goes through a selfdiagnostic test. It is assumed that the sample is available in a suitable, compatible buffer such as 20 mm trishcl, ph 8. Us environmental protection agency office of pesticide programs. Press the mode key until ph is displayed on the right side of primary top. Solutions with a lower ph are termed acidic and solutions with a higher ph are termed alkaline. This sop applies to xxxx cheese produced at the insert company name here and all employees that are involved in the production of this cheese. Select an area and learn more about our wide range of products and applications for weighing, measuring and analyzing. After accepting a good calibration, navigate to the glp file and check the ph slope and slope %. Both sources of variation are avoided by conducting all measurements and standardization at 25ec.
The purpose of this document is to provide a protocol for collecting. Sop for calibration of ph meter and product ph measurement calibration of ph meter sausage maker or designee will calibrate the ph meter prior to use. The procedure and accompanying forms are listed below. Rinse the electrode with deionized water and blot dry with kimwipes. Validation and calibration of analytical instruments. Fill out chemical waste form using the drs website online. Ketika angka ph yang ditampilkan sudah stabil dan tulisan ready muncul, tekan tombol enter untuk menyimpan hasil kalibrasi buffer ph 7. Because the cell has high internal resistance, the ph meter must have a very high input impedance. The ph meter objective to describe the procedure for operation of ph meter scope this sop is applicable for the operation of ph meter responsibility officer. Instruments that will not accept three standards will need to be recalibrated if the water samples ph is outside the initial calibration range described by the two standards. This installment of the series is dedicated to the discussion of the sop on sops and of the. Operation instruction for ph meter model bvcftr document number.
Record all the observation in ph calibration record. Signature on file george mathieus, bureau chief, wqpb date signature on file rosie sada, environmental program manager, wqpb date signature on file prepared by. Standard operating procedures for measuring ph, conductivity. Use and maintenance of a ph meter frederick national laboratory. Standard operating procedure water quality assessment process. If e 4 is flashing, ensure that the correct buffer solution is being used. Standard operating procedure water quality assessment process and methods appendix a to 303d 2000 2004 approvals. Figure 21 shows separate measuring and reference electrodes.
Sop for calibration of ph meter and product ph measurement. With the new fiveeasy and fiveeasyplus bench line instruments for ph and conductivity measurement, we wish to simplify your measuring process and your workflows. Procedures for chainofcustody, sop development, and. Anion exchange chromatography of green fluorescent. Dec 17, 2015 19 applications of ph meter for the diagnosis of various disorders in human body agriculture brewing corrosion prevention dyeing jam and jelly manufacturing printing pharmaceuticals 20. The electrode holder can be mounted on either side of the meter. Use of a superseded or obsolete document is prohibited. If the slope drops below 53, the sensor should be reconditioned and recalibrated. Find ph meter sop related suppliers, manufacturers, products and specifications on globalspec a trusted source of ph meter sop information. The calibration procedure for the jenway 3505 ph meter. The convenient pullout, quickreference card keeps keypad functions at your fingertips.
Again click record to sop recording to the data file. Verification and adjustment of digital conductivity meter and measuring water conductivity. Us environmental protection agency office of pesticide. For greatest accuracy, it is recommended that the instrument be calibrated. The standard operating procedures sops for instruments, include the standard protocol to be followed for the proper and efficient functioning of instruments during the operation by students, faculty, lab technicians and other researchers of the university college. If you are continuing the process of collecting data i. The core document contains the introduction and general forms for level i and ii of. According to iso 9001, i have to calibrate and verify my balance at specified intervals. Mettler toledo is a global provider of precision instruments and services for professional use.
The inf file must be installed to use the meter manual control from a pc. Wait until the meter returns to the normal operating mode to ensure that the calibration process was successful. Standard operating procedures sops for balance testing. Sample collection and preparation is described elsewhere. To calibrate and use a ph meter, prepare a neutral buffer with a ph of 7 and a second buffer with a ph of 4 for acidic samples or 9. The custom liquid crystal display simultaneously shows temperature compensated ph readings and the manually set temperature. P01002a cleaning and preventative maintenance procedures for jenway ph electrodes introduction analysts frequently encounter ph measurement problems caused by poor electrode performance. Model 350 ph meter operating instructions introduction the model 350 is a general purpose hand held ph meter offering a 1 or 2 point calibration and automatic buffer recognition with manual override.
Diagnosis and rectification of these problems can be time consuming, leading to poor analytical results and inefficient. Standard operating procedures for balance testing of sensitivity, repeatability and eccentricity testing your balance too much or not enough. Report sop ph meter please fill this form, we will try to respond as soon as possible. Scope this standard operating procedure sop applies to the staff and students making use of the hanna portable ph meter hi 9025 in the laboratories of the pharmacy department, university of malta. Purpose to outline the procedure for operation of typical ph meter in the traces centre and the undergraduate laboratories. Missouri state university and ozarks environmental and water. Wait 30 seconds and press the confirmation key to confirm the first buffer. Conductance, ph, and chloride using the ysi professional plus handheld multiparameter meter missouri state university and ozarks environmental and water. Technical note ph calibration procedure for optimal. Procedures for chainofcustody, sop development, and common field parameter measurements do, ph, chlorine, temperature office of environmental laboratory.
Standard operating procedure for electrometric ph 1. Cleaning and preventative maintenance procedures for. Standard operating procedure production of pasteurized milk. Use the procedures recommended by manufacturer of equipment this will vary, but a general version is shown below. The true value of the sop on sops is often underestimated.
If the lcd then displays ur, the electrode is not properly connected, it is faulty, the temperature sensor is faulty, or there is an open circuit. This method is suitable for the determination of ph in potable. Rinse the electrode with some ph 7 solution or clean water. When the ph meter is in use for longer than 3 hours, the ph of the third buffer shall be checked once every three hours. Standard operating procedure use and maintenance of a ph meter document id. Sop for access control system, numbering and usage. Sop 52 verification and adjustment of digital conductivity. For ph meter unit 530 and 530p, check the level of the 3m kcl fill solution every day when in use to ensure that it is within 25 mm of the filling opening. Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user remove gloves prior to entering commands via the computer keyboard or mouse. Mettler toledo sevencompact ph meter s220 user manual. Mettlertoledo operating instructions seveneasy ph meter s20.
Next, put a clean electrode in the neutral buffer and press the measure or calibrate button. Standard operating procedures for water qualit monitoring and. Check the ph of samples using the water sample ph meter to ensure that the sample ph is. Sop for assigning of identification numbers to equipments, subequipments. Calibrationverification procedures and forms research. Validation and calibration of analytical instruments ad. If necessary allow several minutes for temperature equilibration. This specification covers the procedure for testing the conductivity and resistivity of the water stored and used in the laboratory for gravimetric. You can also perform a 1point calibration, but make sure that the buffer value is close to the sample value you are measuring.